Coding

Part:BBa_K4853004:Design

Designed by: Bhavya Guduru   Group: iGEM23_Virginia   (2023-10-12)


Transdermal delivery chaperone


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

While TD-1 exists in the iGEM registry, this part was codon optimized for E. coli BL21(DE3) via IDT Codon Optimization Tool.


Source

TD-1 sequence was found in paper titled: Enhanced Transdermal Delivery of Epidermal Growth Factor Facilitated by Dual Peptide Chaperone Motifs1.

1Jin, P., Li, F., Ruan, R., Zhang, L., Man, N., Hu, Y., Zhou, W., & Wen, L. (2014). Enhanced transdermal delivery of epidermal growth factor facilitated by dual peptide chaperone motifs. Protein & Peptide Letters, 21(6), 550–555.

References